Example sentences for: n pr

How can you use “n pr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • However, there is one major difference: pR in OG exhibits an upshifted, doublet C=N Schiff base band consisting of two components of nearly-equal intensity.

  • This would be consistent with a proposed mechanism for fast H +release that has been observed above pH 10 in the bR mutant E194Q [ 14 ] . In this mutant, Asp-85 was detected by low-temperature infrared difference spectroscopy to be deprotonated only in the N intermediate, and not in M [ 15 ] . It is not clear yet whether Asp-85 deprotonation in an N-like state could account for the proton-release kinetics of pR (Fig.

  • However, in bR the M intermediate is detectable at low and high pHs, but has a longer lifetime at higher pH due to a long-lived equilibrium between M and another intermediate, N [ 13 ] . It seems likely that an N intermediate of pR is similarly in equilibrium with its M intermediate, based on the fact that the transient positive (400 nm) absorbance increase is smaller than the negative 500 nm bleach, and the time course at 500 nm shows a partial return to baseline on a timescale of ~50 μs.

  • There is a clear kinetic correlation between M (and/or N) intermediate formation and fast H +release in pR.

  • pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid


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