Words similar to n pr

Example sentences for: n pr

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This would be consistent with a proposed mechanism for fast H +release that has been observed above pH 10 in the bR mutant E194Q [ 14 ] . In this mutant, Asp-85 was detected by low-temperature infrared difference spectroscopy to be deprotonated only in the N intermediate, and not in M [ 15 ] . It is not clear yet whether Asp-85 deprotonation in an N-like state could account for the proton-release kinetics of pR (Fig.
However, in bR the M intermediate is detectable at low and high pHs, but has a longer lifetime at higher pH due to a long-lived equilibrium between M and another intermediate, N [ 13 ] . It seems likely that an N intermediate of pR is similarly in equilibrium with its M intermediate, based on the fact that the transient positive (400 nm) absorbance increase is smaller than the negative 500 nm bleach, and the time course at 500 nm shows a partial return to baseline on a timescale of ~50 μs.
The likely spectral overlap between pR and its own postulated N photoproduct complicates the determination of amounts of each that are present.
4, along with previously published time traces at 600 nm [ 1 ] : the resting state (pR), M, N and O. The resting state, with an absorbance maximum of 546 nm (see above), provides the baseline spectrum for the difference time courses reported.
The H +signals from pR measured with Cresol Red occur on a time scale similar to that assignable to M and N decay, returning to baseline about 1 s after photolysis.